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Accurate non-invasive image-based cytotoxicity assays for cultured cells.

ABSTRACT:

Background

The CloneSelect Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay.

Results

Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay.

Conclusions

This new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested.

SUBMITTER: Marques-Gallego P

PROVIDER: S-EPMC2906423 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Accurate non-invasive image-based cytotoxicity assays for cultured cells.

Marqués-Gallego Patricia P den Dulk Hans H Backendorf Claude C Brouwer Jaap J Reedijk Jan J Burke Julian F JF

BMC biotechnology 20100617

<h4>Background</h4>The CloneSelect Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay.<h4>Results</h4>Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the M ...[more]

PMID: 20565747

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