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Precise mapping of RNA tertiary structure via nanometer distance measurements with double electron-electron resonance spectroscopy.

ABSTRACT: Divalent metal (Mg(2+)) ion-dependent folding of the hammerhead ribozyme from Schistosoma mansoni was monitored with double electron-electron resonance (DEER) pulsed electron paramagnetic resonance spectroscopy by measuring nanometer-scale distances between paramagnetic spin-labels attached to the RNA. DEER measurements detect global folding of the ribozyme with excellent agreement between predictions from experimental, modeled, and crystallographic measurements. These measurements demonstrate the use of DEER spectroscopy as a tool for structural analysis of complex RNAs.

SUBMITTER: Kim NK

PROVIDER: S-EPMC2908488 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Precise mapping of RNA tertiary structure via nanometer distance measurements with double electron-electron resonance spectroscopy.

Kim Nak-Kyoon NK Bowman Michael K MK DeRose Victoria J VJ

Journal of the American Chemical Society 20100701 26

Divalent metal (Mg(2+)) ion-dependent folding of the hammerhead ribozyme from Schistosoma mansoni was monitored with double electron-electron resonance (DEER) pulsed electron paramagnetic resonance spectroscopy by measuring nanometer-scale distances between paramagnetic spin-labels attached to the RNA. DEER measurements detect global folding of the ribozyme with excellent agreement between predictions from experimental, modeled, and crystallographic measurements. These measurements demonstrate t ...[more]

PMID: 20557039

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