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Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

ABSTRACT: A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

SUBMITTER: Gao L

PROVIDER: S-EPMC2909105 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy.

Gao Liang L Kester Robert T RT Hagen Nathan N Tkaczyk Tomasz S TS

Optics express 20100701 14

A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 x 285 x 60 (x, y, lambda). The spatial resolution is approximately 0.45 microm with a FOV of 100 x 100 microm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval approximately 3.3 nm. The channel's spectral resolution is approximately 8nm. The spectral imaging results demonstra ...[more]

PMID: 20639917

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