Ontology highlight
ABSTRACT:
SUBMITTER: Patel K
PROVIDER: S-EPMC2910061 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Patel Katan K Dickson Jacqueline J Din Shahida S Macleod Kenneth K Jodrell Duncan D Ramsahoye Bernard B
Nucleic acids research 20100325 13
5-Aza-2'-deoxycytidine (5-aza-dC) is a nucleoside analogue with cytotoxic and DNA demethylating effects. Here we show that 5-aza-dC induces the proteasomal degradation of free (non-chromatin bound) DNMT1 through a mechanism which is dependent on DNA synthesis and the targeting of incorporated 5-aza-dC residues by DNMT1 itself. Thus, 5-aza-dC induces Dnmt1 degradation in wild-type mouse ES cells, but not in Dnmt [3a(-/-), 3b(-/-)] mouse ES cells which express Dnmt1 but lack DNA methylation (<0.7% ...[more]