Ontology highlight
ABSTRACT:
SUBMITTER: Sivalingam J
PROVIDER: S-EPMC2911251 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Sivalingam Jaichandran J Krishnan Shruti S Ng Wai Har WH Lee Sze Sing SS Phan Toan Thang TT Kon Oi Lian OL
Molecular therapy : the journal of the American Society of Gene Therapy 20100427 7
Biosafety and efficacy considerations that impede clinical application of gene therapy could be addressed by nonviral ex vivo cell therapy, utilizing transgenic cells that have been comprehensively pre-evaluated for genotoxic potential and transgene expression. We evaluated the genotoxic potential of phiC31 bacteriophage integrase-mediated transgene integration in cord-lining epithelial cells (CLECs) readily cultured from the outer membrane of human umbilical cords, by sequencing and mapping int ...[more]