Crystallizing transmembrane peptides in lipidic mesophases.
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ABSTRACT:
SUBMITTER: Hofer N
PROVIDER: S-EPMC2913208 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Publications
Crystallizing transmembrane peptides in lipidic mesophases.
Höfer Nicole N Aragão David D Caffrey Martin M
Biophysical journal 20100801 3
Structure determination of membrane proteins by crystallographic means has been facilitated by crystallization in lipidic mesophases. It has been suggested, however, that this so-called in meso method, as originally implemented, would not apply to small protein targets having </=4 transmembrane crossings. In our study, the hypothesis that the inherent flexibility of the mesophase would enable crystallogenesis of small proteins was tested using a transmembrane pentadecapeptide, linear gramicidin, ...[more]
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