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Clinically relevant doses of chemotherapy agents reversibly block formation of glioblastoma neurospheres.

ABSTRACT: Glioblastoma patients have a poor prognosis, even after surgery, radiotherapy, and chemotherapy with temozolomide or 1,3-bis(2-chloroethy)-1-nitrosourea. We developed an in vitro recovery model using neurosphere cultures to analyze the efficacy of chemotherapy treatments, and tested whether glioblastoma neurosphere-initiating cells are resistant. Concentrations of chemotherapy drugs that inhibit neurosphere formation are similar to clinically relevant doses. Some lines underwent a transient cell cycle arrest and a robust recovery of neurosphere formation. These results indicate that glioblastoma neurospheres can regrow after treatment with chemotherapy drugs. This neurosphere recovery assay will facilitate studies of chemo-resistant subpopulations and methods to enhance glioblastoma therapy.

SUBMITTER: Mihaliak AM

PROVIDER: S-EPMC2914162 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Clinically relevant doses of chemotherapy agents reversibly block formation of glioblastoma neurospheres.

Mihaliak Alicia M AM Gilbert Candace A CA Li Li L Daou Marie-Claire MC Moser Richard P RP Reeves Andrew A Cochran Brent H BH Ross Alonzo H AH

Cancer letters 20101001 2

Glioblastoma patients have a poor prognosis, even after surgery, radiotherapy, and chemotherapy with temozolomide or 1,3-bis(2-chloroethy)-1-nitrosourea. We developed an in vitro recovery model using neurosphere cultures to analyze the efficacy of chemotherapy treatments, and tested whether glioblastoma neurosphere-initiating cells are resistant. Concentrations of chemotherapy drugs that inhibit neurosphere formation are similar to clinically relevant doses. Some lines underwent a transient cell ...[more]

PMID: 20435409

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