Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
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ABSTRACT:
SUBMITTER: Lee X
PROVIDER: S-EPMC2916419 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
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Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
Lee Xiaoyun X Fox Aine A Sufrin Janice J Henry Hugues H Majcherczyk Paul P Haas Dieter D Reimmann Cornelia C
Journal of bacteriology 20100611 16
L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction. ...[more]
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