Cell-based quantification of chronic wasting disease prions.
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ABSTRACT:
SUBMITTER: Bian J
PROVIDER: S-EPMC2916541 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Publications
Cell-based quantification of chronic wasting disease prions.
Bian Jifeng J Napier Dana D Khaychuck Vadim V Angers Rachel R Graham Catherine C Telling Glenn G
Journal of virology 20100602 16
Cell-based measurement of prion infectivity is currently restricted to experimental strains of mouse-adapted scrapie. Having isolated cell cultures with susceptibility to prions from diseased elk, we describe a modification of the scrapie cell assay allowing evaluation of prions causing chronic wasting disease, a naturally occurring transmissible spongiform encephalopathy. We compare this cervid prion cell assay to bioassays in transgenic mice, the only other existing method for quantification, ...[more]
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