Ontology highlight
ABSTRACT:
SUBMITTER: Polley SD
PROVIDER: S-EPMC2916605 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Polley Spencer D SD Mori Yasuyoshi Y Watson Julie J Perkins Mark D MD González Iveth J IJ Notomi Tsugunori T Chiodini Peter L PL Sutherland Colin J CJ
Journal of clinical microbiology 20100616 8
Loop-mediated isothermal amplification (LAMP) of DNA offers the ability to detect very small quantities of pathogen DNA following minimal tissue sample processing and is thus an attractive methodology for point-of-care diagnostics. Previous attempts to diagnose malaria by the use of blood samples and LAMP have targeted the parasite small-subunit rRNA gene, with a resultant sensitivity for Plasmodium falciparum of around 100 parasites per microl. Here we describe the use of mitochondrial targets ...[more]