Ontology highlight
ABSTRACT:
SUBMITTER: Nilsson D
PROVIDER: S-EPMC2916883 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Nilsson Daniel D Gunasekera Kapila K Mani Jan J Osteras Magne M Farinelli Laurent L Baerlocher Loic L Roditi Isabel I Ochsenreiter Torsten T
PLoS pathogens 20100805 8
Trans-splicing of leader sequences onto the 5'ends of mRNAs is a widespread phenomenon in protozoa, nematodes and some chordates. Using parallel sequencing we have developed a method to simultaneously map 5'splice sites and analyze the corresponding gene expression profile, that we term spliced leader trapping (SLT). The method can be applied to any organism with a sequenced genome and trans-splicing of a conserved leader sequence. We analyzed the expression profiles and splicing patterns of blo ...[more]