Ontology highlight
ABSTRACT:
SUBMITTER: Madsen JA
PROVIDER: S-EPMC2917496 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Madsen James A JA Boutz Daniel R DR Brodbelt Jennifer S JS
Journal of proteome research 20100801 8
Ultraviolet photodissociation (UVPD) at 193 nm was implemented on a linear ion trap mass spectrometer for high-throughput proteomic workflows. Upon irradiation by a single 5 ns laser pulse, efficient photodissociation of tryptic peptides was achieved with production of a, b, c, x, y, and z sequence ions, in addition to immonium ions and v and w side-chain loss ions. The factors that influence the UVPD mass spectra and subsequent in silico database searching via SEQUEST were evaluated. Peptide se ...[more]