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Identification of a transcriptional fingerprint of estrogen exposure in rainbow trout liver.

ABSTRACT: The goal of this study was to identify a set of hepatic genes regulated by ligand-dependent activation of the estrogen receptor in juvenile rainbow trout (Oncorhynchus mykiss). A custom rainbow trout oligo DNA microarray, which contains probes targeting approximately 1450 genes relevant to carcinogenesis, toxicology, endocrinology, and stress physiology was utilized to identify transcriptional fingerprints of in vivo dietary exposure to 17 beta-estradiol (E2), tamoxifen (TAM), estradiol + tamoxifen (E2 + TAM), diethylstilbestrol (DES), dehydroepiandrosterone (DHEA), dihydrotestosterone (DHT), and cortisol (CORT). Estrogen exposure altered the expression of up to 49 genes involved in reproduction, immune response, cell growth, transcriptional regulation, protein synthesis and modification, drug metabolism, redox regulation, and signal transduction. E2, DES, and DHEA regulated 18 genes in common, mostly those associated with vitellogenesis, cell proliferation, and signal transduction. Interestingly, DHEA uniquely regulated several complement component genes of importance to immune response. While the effect of TAM on E2-induced changes in gene expression was mostly antagonistic, TAM alone increased expression of VTG1 and other genes associated with egg development and immune response. Few genes responded to CORT treatment, and DHT significantly altered expression of only one gene targeted by the OSUrbt array. Hierarchical cluster and principal components analyses revealed distinct patterns of gene expression corresponding to estrogens and non-estrogens, though unique patterns could also be detected for individual chemicals. A set of estrogen-responsive genes has been identified that can serve as a biomarker of environmental exposure to xenoestrogens.

SUBMITTER: Benninghoff AD

PROVIDER: S-EPMC2917912 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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Identification of a transcriptional fingerprint of estrogen exposure in rainbow trout liver.

Benninghoff Abby D AD Williams David E DE

Toxicological sciences : an official journal of the Society of Toxicology 20070906 1

The goal of this study was to identify a set of hepatic genes regulated by ligand-dependent activation of the estrogen receptor in juvenile rainbow trout (Oncorhynchus mykiss). A custom rainbow trout oligo DNA microarray, which contains probes targeting approximately 1450 genes relevant to carcinogenesis, toxicology, endocrinology, and stress physiology was utilized to identify transcriptional fingerprints of in vivo dietary exposure to 17 beta-estradiol (E2), tamoxifen (TAM), estradiol + tamoxi ...[more]

PMID: 17823450

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Project description:Estrogens are critical hormones involved in reproduction and need to bind to estrogen receptors in target organs for biological activity. Fishes have two distinct estrogen receptor subtypes, alpha (?) and beta (?), with variable combinations of additional isoforms of each subtype dependent on the history of genome duplication within a taxon. The comparative expression patterns of estrogen receptor isoforms during the female reproductive cycle will provide important insights into the unique function and importance of each. The purpose of this study was to measure the mRNAs for the four estrogen receptor isoforms (er?1, er?2, er?1, er?2) in the liver and ovary of adult, female rainbow trout over the course of an annual reproductive cycle. The expression of estrogen receptor mRNA isoforms was measured by quantitative real-time RT-PCR. Several reproductive indices (gonadosomatic index, maximum oocyte diameter, plasma estradiol-17?, plasma vitellogenin, and ovulation) were also quantified for comparison and used in a correlation analysis to examine any inter-relationships. Of the four isoforms, the expression of er?1 was highest in the liver, and had a significant positive correlation with liver er?1 expression. Liver expression of er?2 mRNA was the lowest, but showed a significant positive correlation with maximum oocyte diameter in the ovary. The pattern of the er? isoforms in liver was one of initially elevated mRNA expression followed by a gradual decrease as reproductive development proceeded. In the ovary the er?1 isoform had the highest mRNA expression of all estrogen receptor isoforms, at the beginning of the reproductive cycle, but then decreased afterward. Both ovarian er? isoforms had a significant positive correlation with one another. In contrast, er?2 mRNA expression showed a high maximum level in the ovary near the end of the cycle along with a significant positive correlation with plasma estradiol-17? levels; the highest gonadosomatic indices, maximum oocyte diameter, and vitellogenin levels occurred then too.

Dataset Information

Identification of a transcriptional fingerprint of estrogen exposure in rainbow trout liver.

Publications

Identification of a transcriptional fingerprint of estrogen exposure in rainbow trout liver.

Similar Datasets

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