Production of infectious hepatitis C virus in tissue culture from a cloned viral genome.
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ABSTRACT:
SUBMITTER: Wakita T
PROVIDER: S-EPMC2918402 | biostudies-literature | 2005 Jul
REPOSITORIES: biostudies-literature
Publications
Production of infectious hepatitis C virus in tissue culture from a cloned viral genome.
Wakita Takaji T Pietschmann Thomas T Kato Takanobu T Date Tomoko T Miyamoto Michiko M Zhao Zijiang Z Murthy Krishna K Habermann Anja A Kräusslich Hans-Georg HG Mizokami Masashi M Bartenschlager Ralf R Liang T Jake TJ
Nature medicine 20050612 7
Hepatitis C virus (HCV) infection causes chronic liver diseases and is a global public health problem. Detailed analyses of HCV have been hampered by the lack of viral culture systems. Subgenomic replicons of the JFH1 genotype 2a strain cloned from an individual with fulminant hepatitis replicate efficiently in cell culture. Here we show that the JFH1 genome replicates efficiently and supports secretion of viral particles after transfection into a human hepatoma cell line (Huh7). Particles have ...[more]
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