Visible fluorescence detection of type III protein secretion from bacterial pathogens.
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ABSTRACT:
SUBMITTER: Yount JS
PROVIDER: S-EPMC2919334 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Publications
Visible fluorescence detection of type III protein secretion from bacterial pathogens.
Yount Jacob S JS Tsou Lun K LK Dossa Paul D PD Kullas Amy L AL van der Velden Adrianus W M AW Hang Howard C HC
Journal of the American Chemical Society 20100601 24
Type III protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III pr ...[more]
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2016-02-25 | PXD003113 | Pride
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