The Mutyh base excision repair gene influences the inflammatory response in a mouse model of ulcerative colitis.

ABSTRACT:

Background

The Mutyh DNA glycosylase is involved in the repair of oxidized DNA bases. Mutations in the human MUTYH gene are responsible for colorectal cancer in familial adenomatous polyposis. Since defective DNA repair genes might contribute to the increased cancer risk associated with inflammatory bowel diseases, we compared the inflammatory response of wild-type and Mutyh(-/-) mice to oxidative stress.

Methodology/principal findings

The severity of colitis, changes in expression of genes involved in DNA repair and inflammation, DNA 8-oxoguanine levels and microsatellite instability were analysed in colon of mice treated with dextran sulfate sodium (DSS). The Mutyh(-/-) phenotype was associated with a significant accumulation of 8-oxoguanine in colon DNA of treated mice. A

SUBMITTER: Casorelli I 

PROVIDER: S-EPMC2919403 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature