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CHK2-independent induction of telomere dysfunction checkpoints in stem and progenitor cells.

ABSTRACT: Telomere shortening limits the proliferation of primary human fibroblasts by the induction of senescence, which is mediated by ataxia telangiectasia mutated-dependent activation of p53. Here, we show that CHK2 deletion impairs the induction of senescence in mouse and human fibroblasts. By contrast, CHK2 deletion did not improve the stem-cell function, organ maintenance and lifespan of telomere dysfunctional mice and did not prevent the induction of p53/p21, apoptosis and cell-cycle arrest in telomere dysfunctional progenitor cells. Together, these results indicate that CHK2 mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level in vivo.

SUBMITTER: Nalapareddy K

PROVIDER: S-EPMC2920444 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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CHK2-independent induction of telomere dysfunction checkpoints in stem and progenitor cells.

Nalapareddy Kodandaramireddy K Choudhury Aaheli Roy AR Gompf Anne A Ju Zhenyu Z Ravipati Satyavani S Leucht Thomas T Lechel André A Rudolph K Lenhard KL

EMBO reports 20100625 8

Telomere shortening limits the proliferation of primary human fibroblasts by the induction of senescence, which is mediated by ataxia telangiectasia mutated-dependent activation of p53. Here, we show that CHK2 deletion impairs the induction of senescence in mouse and human fibroblasts. By contrast, CHK2 deletion did not improve the stem-cell function, organ maintenance and lifespan of telomere dysfunctional mice and did not prevent the induction of p53/p21, apoptosis and cell-cycle arrest in tel ...[more]

PMID: 20577265

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