Ontology highlight
ABSTRACT:
SUBMITTER: Kim Y
PROVIDER: S-EPMC2921712 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Kim Yumi Y Holland Andrew J AJ Lan Weijie W Cleveland Don W DW
Cell 20100801 3
Opposing roles of Aurora kinases and protein phosphatase 1 (PP1) during mitosis have long been suggested. Here, we demonstrate that Aurora kinases A and B phosphorylate a conserved residue on the kinetochore motor CENP-E. PP1 binds CENP-E via a motif overlapping this phosphorylation site and binding is disrupted by Aurora phosphorylation. Phosphorylation of CENP-E by the Auroras is enriched at spindle poles, disrupting binding of PP1 and reducing CENP-E's affinity for individual microtubules. Th ...[more]