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Phosphorylation stabilizes Nanog by promoting its interaction with Pin1.

ABSTRACT: Embryonic stem cells (ESCs) can undergo unlimited self-renewal and retain the pluripotency to differentiate into all cell types in the body, thus holding great promise as a renewable source of cells for human therapy. The mechanisms that maintain self-renewal of ESCs remain unclear. Here we show that Nanog, a transcription factor crucial for the self-renewal of ESCs, is phosphorylated at multiple Ser/Thr-Pro motifs. This phosphorylation promotes the interaction between Nanog and the prolyl isomerase Pin1, leading to Nanog stabilization by suppressing its ubiquitination. Inhibition of Pin1 activity or disruption of Pin1-Nanog interaction in ESCs suppresses their capability to self-renew and to form teratomas in immunodeficient mice. Therefore, in addition to the stringent transcriptional regulation of Nanog, the expression level of Nanog is also modulated by posttranslational mechanisms.

SUBMITTER: Moretto-Zita M

PROVIDER: S-EPMC2922169 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Phosphorylation stabilizes Nanog by promoting its interaction with Pin1.

Moretto-Zita Matteo M Jin Hua H Shen Zhouxin Z Zhao Tongbiao T Briggs Steven P SP Xu Yang Y

Proceedings of the National Academy of Sciences of the United States of America 20100709 30

Embryonic stem cells (ESCs) can undergo unlimited self-renewal and retain the pluripotency to differentiate into all cell types in the body, thus holding great promise as a renewable source of cells for human therapy. The mechanisms that maintain self-renewal of ESCs remain unclear. Here we show that Nanog, a transcription factor crucial for the self-renewal of ESCs, is phosphorylated at multiple Ser/Thr-Pro motifs. This phosphorylation promotes the interaction between Nanog and the prolyl isome ...[more]

PMID: 20622153

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