Ontology highlight
ABSTRACT:
SUBMITTER: Evseenko D
PROVIDER: S-EPMC2922221 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Evseenko Denis D Zhu Yuhua Y Schenke-Layland Katja K Kuo Jeffrey J Latour Brooke B Ge Shundi S Scholes Jessica J Dravid Gautam G Li Xinmin X MacLellan W Robb WR Crooks Gay M GM
Proceedings of the National Academy of Sciences of the United States of America 20100719 31
Our understanding of how mesodermal tissue is formed has been limited by the absence of specific and reliable markers of early mesoderm commitment. We report that mesoderm commitment from human embryonic stem cells (hESCs) is initiated by epithelial-to-mesenchymal transition (EMT) as shown by gene expression profiling and by reciprocal changes in expression of the cell surface proteins, EpCAM/CD326 and NCAM/CD56. Molecular and functional assays reveal that the earliest CD326-CD56+ cells, generat ...[more]