Accumulative difference image protocol for particle tracking in fluorescence microscopy tested in mouse lymphonodes.

ABSTRACT: The basic research in cell biology and in medical sciences makes large use of imaging tools mainly based on confocal fluorescence and, more recently, on non-linear excitation microscopy. Substantially the aim is the recognition of selected targets in the image and their tracking in time. We have developed a particle tracking algorithm optimized for low signal/noise images with a minimum set of requirements on the target size and with no a priori knowledge of the type of motion. The image segmentation, based on a combination of size sensitive filters, does not rely on edge detection and is tailored for targets acquired at low resolution as in most of the in-vivo studies. The particle tracking is performed by building, from a stack of Accumulative Difference Images, a single 2D image in whic

SUBMITTER: Villa CE 

PROVIDER: S-EPMC2923183 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature