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Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions.


ABSTRACT: In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and is essential in the signal-emitting cells for Jag1 to activate Notch signalling. In zebrafish, loss- and gain-of-function analyses showed that Mib-Jag1-Notch signalling favours the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. This leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signalling-mediated cell-fate determination.

SUBMITTER: Yamamoto M 

PROVIDER: S-EPMC2927672 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions.

Yamamoto Mai M   Morita Ryoko R   Mizoguchi Takamasa T   Matsuo Hiromi H   Isoda Miho M   Ishitani Tohru T   Chitnis Ajay B AB   Matsumoto Kunihiro K   Crump J Gage JG   Hozumi Katsuto K   Yonemura Shigenobu S   Kawakami Koichi K   Itoh Motoyuki M  

Development (Cambridge, England) 20100623 15


In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiqui  ...[more]

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