Explant outgrowth, propagation and characterization of human pericytes.
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ABSTRACT:
SUBMITTER: Maier CL
PROVIDER: S-EPMC2928225 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Publications
Explant outgrowth, propagation and characterization of human pericytes.
Maier Cheryl L CL Shepherd Benjamin R BR Yi Tai T Pober Jordan S JS
Microcirculation (New York, N.Y. : 1994) 20100701 5
<h4>Objective</h4>Pericytes are critical cellular components of the microvasculature that play a major role in vascular development and pathologies, yet their study has been hindered by lack of a standardized method for their isolation and growth. Here we report a method for culturing human pericytes from a readily available tissue source, placenta, and provide a thorough characterization of resultant cell populations.<h4>Methods</h4>We developed an optimized protocol for obtaining pericytes by ...[more]
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