A method to site-specifically introduce methyllysine into proteins in E. coli.
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ABSTRACT:
SUBMITTER: Ai HW
PROVIDER: S-EPMC2928331 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Publications
A method to site-specifically introduce methyllysine into proteins in E. coli.
Ai Hui-Wang HW Lee Jae Wook JW Schultz Peter G PG
Chemical communications (Cambridge, England) 20100622 30
A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins. ...[more]
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