Subtype identification and functional characterization of ryanodine receptors in rat cerebral artery myocytes.
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ABSTRACT:
SUBMITTER: Vaithianathan T
PROVIDER: S-EPMC2928634 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
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Subtype identification and functional characterization of ryanodine receptors in rat cerebral artery myocytes.
Vaithianathan Thirumalini T Narayanan Damodaran D Asuncion-Chin Maria T MT Jeyakumar Loice H LH Liu Jianxi J Fleischer Sidney S Jaggar Jonathan H JH Dopico Alejandro M AM
American journal of physiology. Cell physiology 20100505 2
Ryanodine receptors (RyRs) regulate contractility in resistance-size cerebral artery smooth muscle, yet their molecular identity, subcellular location, and phenotype in this tissue remain unknown. Following rat resistance-size cerebral artery myocyte sarcoplasmic reticulum (SR) purification and incorporation into POPE-POPS-POPC (5:3:2; wt/wt) bilayers, unitary conductances of 110 +/- 8, 334 +/- 15, and 441 +/- 27 pS in symmetric 300 mM Cs(+) were usually detected. The most frequent (34/40 bilaye ...[more]
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