Ontology highlight
ABSTRACT:
SUBMITTER: Tupin A
PROVIDER: S-EPMC2928680 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
The EMBO journal 20100618 15
Worldwide spreading of drug-resistant pathogens makes mechanistic understanding of antibiotic action an urgent task. The macrocyclic antibiotic lipiarmycin (Lpm), which is under development for clinical use, inhibits bacterial RNA polymerase (RNAP) by an unknown mechanism. Using genetic and biochemical approaches, we show that Lpm targets the sigma(70) subunit region 3.2 and the RNAP beta' subunit switch-2 element, which controls the clamping of promoter DNA in the RNAP active-site cleft. Lpm ab ...[more]