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Preparation of translationally competent tRNA by direct chemical acylation.

ABSTRACT: Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) using amino acid phosphate esters. The successful implementation of this methodology to full-length suppressor tRNA is described, and it is shown that the derived aminoacyl-tRNA is translationally competent in Xenopus oocytes.

SUBMITTER: Duffy NH

PROVIDER: S-EPMC2929286 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Preparation of translationally competent tRNA by direct chemical acylation.

Duffy Noah H NH Dougherty Dennis A DA

Organic letters 20100901 17

Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) using amino acid phosphate esters. The successful implementation of this methodology to full-length suppressor tRNA is described, and it is shown that the derived aminoacyl-tRNA is translationally compe ...[more]

PMID: 20687605

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