Bipartite adenoviral vector encoding hHGF and hIL-1Ra for improved human islet transplantation.

ABSTRACT:

Purpose

Ex vivo gene therapy can improve the outcome of islet transplantation for treating type I diabetes. Hepatocyte growth factor (HGF) increases beta-cell proliferation and promotes revascularization of islets, while interleukin-1 receptor antagonist (hIL-1Ra) inhibits islet cell apoptosis.

Methods

We constructed Adv-hHGF-hIL-1Ra by cloning hHGF and hIL-1Ra coding sequences and polyA signal under separate CMV promoters in Adenoquick plasmid.

Results

There was dose and time dependent expression of these genes after transduction of Adv-hHGF-hIL-1Ra into human islets. Compared to un-transduced islets, hHGF and hIL-1Ra gene expression at protein levels was more than 60 and 40 times higher at 1,000 MOI, respectively. Transduced islets were viable after incubation with

SUBMITTER: Panakanti R 

PROVIDER: S-EPMC2933074 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature