Lamellar bodies form solid three-dimensional films at the respiratory air-liquid interface.

ABSTRACT: Pulmonary surfactant is essential for lung function. It is assembled, stored and secreted as particulate entities (lamellar body-like particles; LBPs). LBPs disintegrate when they contact an air-liquid interface, leading to an instantaneous spreading of material and a decline in surface tension. Here, we demonstrate that the film formed by the adsorbed material spontaneously segregate into distinct ordered and disordered lipid phase regions under unprecedented near-physiological conditions and, unlike natural surfactant purified from bronchoalveolar lavages, dynamically reorganized into highly viscous multilayer domains with complex three-dimensional topographies. Multilayer domains, in coexistence with liquid phases, showed a progressive stiffening and finally solidification, probably dri

SUBMITTER: Ravasio A 

PROVIDER: S-EPMC2934682 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature