Genetic transformation of HeLa cells by Agrobacterium.
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ABSTRACT:
SUBMITTER: Kunik T
PROVIDER: S-EPMC29349 | biostudies-literature | 2001 Feb
REPOSITORIES: biostudies-literature
Publications
Genetic transformation of HeLa cells by Agrobacterium.
Kunik T T Tzfira T T Kapulnik Y Y Gafni Y Y Dingwall C C Citovsky V V
Proceedings of the National Academy of Sciences of the United States of America 20010130 4
Agrobacterium tumefaciens is a soil phytopathogen that elicits neoplastic growths on the host plant species. In nature, however, Agrobacterium also may encounter organisms belonging to other kingdoms such as insects and animals that feed on the infected plants. Can Agrobacterium, then, also infect animal cells? Here, we report that Agrobacterium attaches to and genetically transforms several types of human cells. In stably transformed HeLa cells, the integration event occurred at the right borde ...[more]
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