Ontology highlight
ABSTRACT:
SUBMITTER: Falk SP
PROVIDER: S-EPMC2935000 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Antimicrobial agents and chemotherapy 20100706 9
We describe a primary high-throughput screen that uses the reporter strain Bacillus subtilis BAU-102 to identify antibiotics that induce autolysis. The screen measures autolysis in terms of the incipient release of recombinant Escherichia coli beta-galactosidase (beta-Gal) from the periplasmic space of B. subtilis owing to a loss of integrity of the cell wall. In a model screen, beta-Gal release values for 79 members of a library consisting of antibiotics and related compounds were collected, so ...[more]