Dataset Information
Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate.
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ABSTRACT:
SUBMITTER: Tian YS
PROVIDER: S-EPMC2935038 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Publications
Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate.
Applied and environmental microbiology 20100702 17
Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance. ...[more]
