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Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate.

ABSTRACT: Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.

SUBMITTER: Tian YS

PROVIDER: S-EPMC2935038 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate.

Tian Yong-Sheng YS Xiong Ai-Sheng AS Xu Jing J Zhao Wei W Gao Feng F Fu Xiao-Yan XY Xu Hu H Zheng Jian-Li JL Peng Ri-He RH Yao Quan-Hong QH

Applied and environmental microbiology 20100702 17

Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance. ...[more]

PMID: 20601515

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