Ontology highlight
ABSTRACT:
SUBMITTER: Jackson AJ
PROVIDER: S-EPMC2935639 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Jackson Abby J AJ Karle Elizabeth M EM Hage David S DS
Analytical biochemistry 20100803 2
This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39 mg protein G/g silica was obtained when using 100 A pore size silica, followed by 33 mg/g for 50 A silica and 9.3-24 mg/g for 300-4000 A silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037 micromol/m(2) being obtained for 4000 A silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunog ...[more]