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Preparation of high-capacity supports containing protein G immobilized to porous silica.


ABSTRACT: This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39 mg protein G/g silica was obtained when using 100 A pore size silica, followed by 33 mg/g for 50 A silica and 9.3-24 mg/g for 300-4000 A silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037 micromol/m(2) being obtained for 4000 A silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunoglobulin G [IgG]), with the highest binding capacities (71-77 mg IgG/g silica) being obtained for 50-100 A silica.

SUBMITTER: Jackson AJ 

PROVIDER: S-EPMC2935639 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Preparation of high-capacity supports containing protein G immobilized to porous silica.

Jackson Abby J AJ   Karle Elizabeth M EM   Hage David S DS  

Analytical biochemistry 20100803 2


This study examined the preparation of high-capacity silica supports containing immobilized protein G. A maximum content of 39 mg protein G/g silica was obtained when using 100 A pore size silica, followed by 33 mg/g for 50 A silica and 9.3-24 mg/g for 300-4000 A silica. The surface coverage of protein G increased with pore size, with a maximum level of 0.037 micromol/m(2) being obtained for 4000 A silica. These supports gave comparable apparent activities (i.e., 30-47% binding to rabbit immunog  ...[more]

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