Characterization of a family GH5 xylanase with activity on neutral oligosaccharides and evaluation as a pulp bleaching aid.
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ABSTRACT:
SUBMITTER: Gallardo O
PROVIDER: S-EPMC2937475 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
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Characterization of a family GH5 xylanase with activity on neutral oligosaccharides and evaluation as a pulp bleaching aid.
Gallardo Oscar O Fernández-Fernández María M Valls Cristina C Valenzuela Susana Valeria SV Roncero M Blanca MB Vidal Teresa T Díaz Pilar P Pastor F I Javier FI
Applied and environmental microbiology 20100723 18
A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for methyl-glucuronic acid side chains for catalysis. The enzyme was evaluated on Eucalyptus kraft pulp, showing its effectiveness as a bleaching aid. ...[more]
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