RNA 5'-triphosphatase activity of the hepatitis E virus helicase domain.
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ABSTRACT:
SUBMITTER: Karpe YA
PROVIDER: S-EPMC2937651 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Publications
RNA 5'-triphosphatase activity of the hepatitis E virus helicase domain.
Karpe Yogesh A YA Lole Kavita S KS
Journal of virology 20100630 18
Hepatitis E virus (HEV) has a positive-sense RNA genome with a 5'-m7G cap. HEV open reading frame 1 (ORF1) encodes a polyprotein with multiple enzyme domains required for replication. HEV helicase is a nucleoside triphosphatase (NTPase) with the ability to unwind RNA duplexes in the 5'-to-3' direction. When incubated with 5'-[gamma-(32)P]RNA and 5'-[alpha-(32)P]RNA, HEV helicase released (32)P only from 5'-[gamma-(32)P]RNA, showing specificity for the gamma-beta-triphosphate bond. Removal of gam ...[more]
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