Ontology highlight
ABSTRACT:
SUBMITTER: Bebenek K
PROVIDER: S-EPMC2938210 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Bebenek Katarzyna K Garcia-Diaz Miguel M Zhou Rui-Zhe RZ Povirk Lawrence F LF Kunkel Thomas A TA
Nucleic acids research 20100430 16
Differences in the substrate specificity of mammalian family X DNA polymerases are proposed to partly depend on a loop (loop 1) upstream of the polymerase active site. To examine if this is the case in DNA polymerase λ (pol λ), here we characterize a variant of the human polymerase in which nine residues of loop 1 are replaced with four residues from the equivalent position in pol β. Crystal structures of the mutant enzyme bound to gapped DNA with and without a correct dNTP reveal that the chang ...[more]