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The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration.

ABSTRACT: The RNA binding protein Larp1 was originally shown to be involved in spermatogenesis, embryogenesis and cell-cycle progression in Drosophila. Our data show that mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4E and is associated with 60S and 80S ribosomal subunits. A reduction in Larp1 expression by siRNA inhibits global protein synthesis rates and results in mitotic arrest and delayed cell migration. Consistent with these data we show that Larp1 protein is present at the leading edge of migrating cells and interacts directly with cytoskeletal components. Taken together, these data suggest a role for Larp1 in facilitating the synthesis of proteins required for cellular remodelling and migration.

SUBMITTER: Burrows C

PROVIDER: S-EPMC2938220 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration.

Burrows Carla C Abd Latip Normala N Lam Sarah-Jane SJ Carpenter Lee L Sawicka Kirsty K Tzolovsky George G Gabra Hani H Bushell Martin M Glover David M DM Willis Anne E AE Blagden Sarah P SP

Nucleic acids research 20100429 16

The RNA binding protein Larp1 was originally shown to be involved in spermatogenesis, embryogenesis and cell-cycle progression in Drosophila. Our data show that mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4E and is associated with 60S and 80S ribosomal subunits. A reduction in Larp1 expression by siRNA inhibits global protein synthesis rates and results in mitotic arrest and delayed cell migration. Consistent with these data we show that Larp ...[more]

PMID: 20430826

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