Project description:The Neotropics contains half of remaining rainforests and Earth's largest reservoir of amphibian biodiversity. However, determinants of Neotropical biodiversity (i.e., vicariance, dispersals, extinctions, and radiations) earlier than the Quaternary are largely unstudied. Using a novel method of ancestral area reconstruction and relaxed Bayesian clock analyses, we reconstructed the biogeography of the poison frog clade (Dendrobatidae). We rejected an Amazonian center-of-origin in favor of a complex connectivity model expanding over the Neotropics. We inferred 14 dispersals into and 18 out of Amazonia to adjacent regions; the Andes were the major source of dispersals into Amazonia. We found three episodes of lineage dispersal with two interleaved periods of vicariant events between South and Central America. During the late Miocene, Amazonian, and Central American-Chocoan lineages significantly increased their diversity compared to the Andean and Guianan-Venezuelan-Brazilian Shield counterparts. Significant percentage of dendrobatid diversity in Amazonia and Chocó resulted from repeated immigrations, with radiations at <10.0 million years ago (MYA), rather than in situ diversification. In contrast, the Andes, Venezuelan Highlands, and Guiana Shield have undergone extended in situ diversification at near constant rate since the Oligocene. The effects of Miocene paleogeographic events on Neotropical diversification dynamics provided the framework under which Quaternary patterns of endemism evolved.
Project description:Tropical forests are disappearing, but we have a limited understanding of the factors driving species coexistence in mammal communities of old-growth forest ecosystems. The total energy that is bound by plants is assumed to be a key factor determining mammalian species richness, but accurately measuring energy flows in complex ecosystems is difficult, and most studies therefore rely on remote-sensing-based surrogates of net primary productivity (NPP). We monitored mammal species richness across three seasons using camera traps on 26 study plots along a forested, elevational gradient from 245 to 3588 m above sea level in southeastern Peru for which a unique dataset on field-measured NPP exists. Using linear-regression models and path analysis, we disentangled the effects of climate and NPP on the diversity of mammals, testing the predictions of the more-individuals hypothesis, stating that energy availability drives the number of individuals and, thus, the number of coexisting species. We compared detailed field measurements of NPP with remote-sensing products (MODIS NPP and MODIS NDVI). Mammal species richness, abundance, and biomass decreased in a negative exponential pattern with elevation. Field-measured data on NPP, which was largely driven by temperature, was a strong predictor of both abundance and species richness, while remotely sensed proxies for NPP failed to accurately predict mammal diversity. Our study underpins the importance of field-based ecosystem data and emphasizes the role of high primary productivity for maintaining diverse mammal communities, which is a particularly pressing issue in light of recent anthropogenic impacts on the Amazonian forest system.
Project description:The Global Panzootic Lineage (GPL) of the pathogenic fungus Batrachochytrium dendrobatidis (Bd) has caused severe amphibian population declines, yet the drivers underlying the high frequency of GPL in regions of amphibian decline are unclear. Using publicly available Bd genome sequences, we identified multiple non-GPL Bd isolates that contain a circular Rep-encoding single stranded DNA-like virus which we named BdDV-1. We further sequenced and constructed genome assemblies with long read sequences to find that the virus is integrated into the nuclear genome in some strains. Attempts to cure virus positive isolates were unsuccessful, however, phenotypic differences between naturally virus positive and virus negative Bd isolates suggested that BdDV-1 decreases the growth of its host in vitro but increases the virulence of its host in vivo. BdDV-1 is the first described CRESS DNA mycovirus associated with hypervirulence with a distribution inversely associated with the emergence of the panzootic lineage.
Project description:The melting of tropical glaciers provides water resources to millions of people, involving social, ecological and economic demands. At present, these water reservoirs are threatened by the accelerating rates of mass loss associated with modern climate changes related to greenhouse gas emissions and ultimately land use/cover change. Until now, the effects of land use/cover change on the tropical Andean glaciers of South America through biomass burning activities have not been investigated. In this study, we quantitatively examine the hypothesis that regional land use/cover change is a contributor to the observed glacier mass loss, taking into account the role of Amazonian biomass burning. We demonstrated here, for the first time, that for tropical Andean glaciers, a massive contribution of black carbon emitted from biomass burning in the Amazon Basin does exist. This is favorable due to its positioning with respect to Amazon Basin fire hot spots and the predominant wind direction during the transition from the dry to wet seasons (Aug-Sep-Oct), when most fire events occur. We investigated changes in Bolivian Zongo Glacier albedo due to impurities on snow, including black carbon surface deposition and its potential for increasing annual glacier melting. We showed that the magnitude of the impact of Amazonian biomass burning depends on the dust content in snow. When high concentration of dust is present (e.g. 100 ppm of dust), the dust absorbs most of the radiation that otherwise would be absorbed by the BC. Our estimations point to a melting factor of 3.3 ± 0.8% for black carbon, and 5.0 ± 1.0% for black carbon in the presence of low dust content (e.g. 10 ppm of dust). For the 2010 hydrological year, we reported an increase in runoff corresponding to 4.5% of the annual discharge during the seasonal peak fire season, which is consistent with our predictions.
Project description:Palaemoncarteri (Gordon, 1935) and Palaemonivonicus (Holthuis, 1950) are morphologically similar species of South American freshwater shrimps. Past studies have questioned the taxonomic status of both species, which are supposed to have partially sympatric geographic distributions in the Amazon basin. We analyzed a 550 bp fragment of the mitochondrial 16S rRNA gene from these Amazonian Palaemon species as well as from 11 palaemonids as the outgroup. Additionally, we checked diagnostic characters of the genus and family as well as other morphological characters that have been little explored before. Palaemoncarteri and Palaemonivonicus are allocated in two sister lineages, with wide genetic divergence and little morphological differentiation. The divergence time between these lineages was estimated as approximately 10 million years ago. Both molecular and morphological data support the taxonomic validity of both Palaemoncarteri and Palaemonivonicus, refuting the hypothesis of synonymy. In addition, a new species, Palaemonyuna sp. n., closely related to Palaemonivonicus, is described. Our findings indicate that these species can be differentiated using the projection of the anterolateral margin and anterolateral spine of the first antennular segment, shape of the rostrum, and relative size of the appendix masculina.
Project description:The fossil ape Lufengpithecus is known from a number of late Miocene sites in Yunnan Province in southern China. Along with other fossil apes from South and Southeast Asia, it is widely considered to be a relative of the extant orangutan, Pongo pygmaeus. It is best represented at the type site of Shihuiba (Lufeng) by several partial to nearly complete but badly crushed adult crania. There is, however, an additional, minimally distorted cranium of a young juvenile from a nearly contemporaneous site in the Yuanmou Basin, which affords the opportunity to better assess the relationships between Lufengpithecus and Pongo. Comparison with similarly aged juvenile skulls of extant great apes reveals no features suggesting clear affinities to orangutans, and instead reveals a morphological pattern largely consistent with a stem member of the hominid (great ape and human) clade. The existence at this time of other hominids in South Asia (Sivapithecus) and Southeast Asia (Khoratpithecus) with clear craniofacial affinities to Pongo suggests both more diversity among Asian Late Miocene apes and more complex patterns of dispersal than previously supposed. Major differences in the associated mammal faunas from the southern China sites and those from South and Southeast Asia are consistent with these findings and suggest more than one dispersal route of apes into East Asia earlier in the Miocene.
Project description:The largest megalake in the geological record formed in Eurasia during the late Miocene, when the epicontinental Paratethys Sea became tectonically-trapped and disconnected from the global ocean. The megalake was characterized by several episodes of hydrological instability and partial desiccation, but the chronology, magnitude and impacts of these paleoenvironmental crises are poorly known. Our integrated stratigraphic study shows that the main desiccation episodes occurred between 9.75 and 7.65 million years ago. We identify four major regressions that correlate with aridification events, vegetation changes and faunal turnovers in large parts of Europe. Our paleogeographic reconstructions reveal that the Paratethys was profoundly transformed during regression episodes, losing ~ 1/3 of the water volume and ~ 70% of its surface during the most extreme events. The remaining water was stored in a central salt-lake and peripheral desalinated basins while vast regions (up to 1.75 million km2) became emergent land, suitable for development of forest-steppe landscapes. The partial megalake desiccations match with climate, food-web and landscape changes throughout Eurasia, although the exact triggers and mechanisms remain to be resolved.
Project description:South America is home to the highest freshwater fish biodiversity on Earth, and the hotspot of species richness is located in the western Amazon basin. The location of this hotspot is enigmatic, as it is inconsistent with the pattern observed in river systems across the world of increasing species richness towards a river's mouth. Here we investigate the role of river capture events caused by Andean mountain building and repeated episodes of flooding in western Amazonia in shaping the modern-day richness pattern of freshwater fishes in South America, and in Amazonia in particular. To this end, we combine a reconstruction of river networks since 80 Ma with a mechanistic model simulating dispersal, allopatric speciation and extinction over the dynamic landscape of rivers and lakes. We show that Andean mountain building and consequent numerous small river capture events in western Amazonia caused freshwater habitats to be highly dynamic, leading to high diversification rates and exceptional richness. The history of marine incursions and lakes, including the Miocene Pebas mega-wetland system in western Amazonia, played a secondary role.
Project description:The Amazonian landscape evolution is the result of the combined effect of Andean tectonism, climate and the Earth's interior dynamics. To reconstruct the landscape evolution and its influence on paleoenvironmental variations within Amazonia since the Oligocene, we conducted numerical experiments that incorporate different surface and geodynamic processes, reproducing many paleogeographic features as inferred from the sedimentary record. We show that the evolution of the drainage pattern gradually reduced the area of sedimentation derived from the Guiana and Brazilian shields while expanded the Andean derived deposits during the Miocene, affecting the nutrient availability. First order biotic habitats were inferred from these paleogeographical reconstructions, showing an eastward expansion of várzea and terra firme forests and consequent retraction of igapó forests, with a millennial-scale reconfiguration of a mosaic of habitats in the lowlands. We conclude that this dynamism probably guided the observed patterns of speciation in the most biodiverse biome on Earth.
Project description:Understanding deep-time marine biodiversity change under the combined effects of climate and connectivity changes is fundamental for predicting the impacts of modern climate change in semi-enclosed seas. We quantify the Late Miocene-Early Pliocene [11.63 to 3.6 million years (Ma)] taxonomic diversity of the Mediterranean Sea for calcareous nannoplankton, dinocysts, foraminifera, ostracods, corals, molluscs, bryozoans, echinoids, fishes, and marine mammals. During this time, marine biota was affected by global climate cooling and the restriction of the Mediterranean's connection to the Atlantic Ocean that peaked with the Messinian salinity crisis. Although the net change in species richness from the Tortonian to the Zanclean varies by group, species turnover is greater than 30% in all cases, reflecting a high degree of reorganization of the marine ecosystem after the crisis. The results show a clear perturbation already in the pre-evaporitic Messinian (7.25 to 5.97 Ma), with patterns differing among groups and subbasins.