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Solid-state NMR characterization of gas vesicle structure.

ABSTRACT: Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-?-?-?-?-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a picture of the overall topology of the vesicle subunit that is consistent with its role in stabilizing an air-water interface.

SUBMITTER: Sivertsen AC

PROVIDER: S-EPMC2941023 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Solid-state NMR characterization of gas vesicle structure.

Sivertsen Astrid C AC Bayro Marvin J MJ Belenky Marina M Griffin Robert G RG Herzfeld Judith J

Biophysical journal 20100901 6

Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-α-β-β-α-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a pictu ...[more]

PMID: 20858439

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