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Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance.

ABSTRACT: We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect the binding of the viral particles to surfaces functionalized with and without antibodies. We show that the intensity of the particle image is related to the mass of the particle, from which we determine the mass and mass distribution of influenza viral particles with a mass detection limit of approximately 1 ag (or 0.2 fg/mm(2)). This work demonstrates a multiplexed method to measure the masses of individual viral particles and to study the binding activity of the viral particles.

SUBMITTER: Wang S

PROVIDER: S-EPMC2941305 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance.

Wang Shaopeng S Shan Xiaonan X Patel Urmez U Huang Xinping X Lu Jin J Li Jinghong J Tao Nongjian N

Proceedings of the National Academy of Sciences of the United States of America 20100826 37

We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect the binding of the viral particles to surfaces functionalized with and without antibodies. We show that the intensity of the particle image is related to the mass of the particle, ...[more]

PMID: 20798340

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