Indirect estimation of the area density of Atg8 on the phagophore.
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ABSTRACT:
SUBMITTER: Xie Z
PROVIDER: S-EPMC2941343 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Publications
Indirect estimation of the area density of Atg8 on the phagophore.
Xie Zhiping Z Nair Usha U Geng Jiefei J Szefler Maciej B MB Rothman Edward D ED Klionsky Daniel J DJ
Autophagy 20090217 2
Atg8 is a ubiquitin-like protein that controls the expansion of the phagophore during autophagosome formation. It is recruited to the phagophore during the expansion stage and released upon the completion of the autophagosome. One possible model explaining the function of Atg8 is that it acts as an adaptor of a coat complex. Here, we tested the coat-adaptor model by estimating the area density of Atg8 molecules on the phagophore. We developed a computational process to simulate the random sectio ...[more]
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