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Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids.

ABSTRACT: Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures.

SUBMITTER: Liu J 

PROVIDER: S-EPMC2941765 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids.

Liu Jia J   Castañeda Carlos A CA   Wilkins Bryan J BJ   Fushman David D   Cropp T Ashton TA  

Bioorganic & medicinal chemistry letters 20100813 19

Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures. ...[more]

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