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Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1?: evidence for opioid-immune crosstalk.

ABSTRACT: Treatment of SK-N-SH cells with morphine and interleukin-1beta (IL-1?) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1? increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1?-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1? and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

SUBMITTER: Mohan S

PROVIDER: S-EPMC2942958 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: evidence for opioid-immune crosstalk.

Mohan Shekher S Davis Randall L RL DeSilva Udaya U Stevens Craig W CW

Journal of neuroimmunology 20100707 1-2

Treatment of SK-N-SH cells with morphine and interleukin-1beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 rece ...[more]

PMID: 20615556

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