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Isolation, cultivation and characterization of adult murine prostate stem cells.


ABSTRACT: The successful isolation and cultivation of prostate stem cells will allow us to study their unique biological properties and their application in therapeutic approaches. Here we describe step-by-step procedures on the basis of previous work in our laboratory for the harvesting of primary prostate cells from adolescent male mice by a modified enzymatic procedure; the isolation of an enriched population of prostate stem cells through cell sorting; and the cultivation of prostate stem cells in vitro and characterization of these cells and their stem-like activity, including in vivo tubule regeneration. Normally, it will take approximately 8 h to harvest prostate cells, isolate the stem cell-enriched population and set up the in vitro sphere assay. It will take up to 8 weeks to analyze the unique properties of the stem cells, including their regenerative capacity in vivo.

SUBMITTER: Lukacs RU 

PROVIDER: S-EPMC2943378 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Isolation, cultivation and characterization of adult murine prostate stem cells.

Lukacs Rita U RU   Goldstein Andrew S AS   Lawson Devon A DA   Cheng Donghui D   Witte Owen N ON  

Nature protocols 20100325 4


The successful isolation and cultivation of prostate stem cells will allow us to study their unique biological properties and their application in therapeutic approaches. Here we describe step-by-step procedures on the basis of previous work in our laboratory for the harvesting of primary prostate cells from adolescent male mice by a modified enzymatic procedure; the isolation of an enriched population of prostate stem cells through cell sorting; and the cultivation of prostate stem cells in vit  ...[more]

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