Ontology highlight
ABSTRACT:
SUBMITTER: Lukacs RU
PROVIDER: S-EPMC2943378 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Lukacs Rita U RU Goldstein Andrew S AS Lawson Devon A DA Cheng Donghui D Witte Owen N ON
Nature protocols 20100325 4
The successful isolation and cultivation of prostate stem cells will allow us to study their unique biological properties and their application in therapeutic approaches. Here we describe step-by-step procedures on the basis of previous work in our laboratory for the harvesting of primary prostate cells from adolescent male mice by a modified enzymatic procedure; the isolation of an enriched population of prostate stem cells through cell sorting; and the cultivation of prostate stem cells in vit ...[more]