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SiRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis.


ABSTRACT: Plant microRNAs (miRNAs) and small interfering RNAs (siRNAs) bear a 2'-O-methyl group on the 3'-terminal nucleotide. This methyl group is post-synthetically added by the methyltransferase protein HEN1 and protects small RNAs from enzymatic activities that target the 3'-OH. A mutagenesis screen for suppressors of the partial loss-of-function hen1-2 allele in Arabidopsis identified second-site mutations that restore miRNA methylation. These mutations affect two subunits of the DNA-dependent RNA polymerase IV (Pol IV), which is essential for the biogenesis of 24 nt endogenous siRNAs. A mutation in RNA-dependent RNA polymerase 2, another essential gene for the biogenesis of endogenous 24-nt siRNAs, also rescued the defects in miRNA methylation of hen1-2, revealing a previously unsuspected, negative influence of siRNAs on HEN1-mediated miRNA methylation. In addition, our findings imply the existence of a negative modifier of HEN1 activity in the Columbia genetic background.

SUBMITTER: Yu B 

PROVIDER: S-EPMC2943618 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis.

Yu Bin B   Bi Liu L   Zhai Jixian J   Agarwal Manu M   Li Shengben S   Wu Qingfa Q   Ding Shou-Wei SW   Meyers Blake C BC   Vaucheret Hervé H   Chen Xuemei X  

Nucleic acids research 20100506 17


Plant microRNAs (miRNAs) and small interfering RNAs (siRNAs) bear a 2'-O-methyl group on the 3'-terminal nucleotide. This methyl group is post-synthetically added by the methyltransferase protein HEN1 and protects small RNAs from enzymatic activities that target the 3'-OH. A mutagenesis screen for suppressors of the partial loss-of-function hen1-2 allele in Arabidopsis identified second-site mutations that restore miRNA methylation. These mutations affect two subunits of the DNA-dependent RNA po  ...[more]

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