Ontology highlight
ABSTRACT:
SUBMITTER: Hinde E
PROVIDER: S-EPMC2944750 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Hinde Elizabeth E Cardarelli Francesco F Digman Michelle A MA Gratton Enrico E
Proceedings of the National Academy of Sciences of the United States of America 20100907 38
No methods proposed thus far have the capability to measure overall molecular flow in the nucleus of living cells. Here, we apply the pair correlation function analysis (pCF) to measure molecular anisotropic diffusion in the interphase nucleus of live cells. In the pCF method, we cross-correlate fluctuations at several distances and locations within the nucleus, enabling us to define migration paths and barriers to diffusion. We use monomeric EGFP as a prototypical inert molecule and measure flo ...[more]