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High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn's disease.


ABSTRACT: AIM:To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis (MAP) in patients with inflammatory bowel disease [Crohn's disease (CD) and ulcerative colitis (UC)]. METHODS:Thirty patients with CD (15 with at least one NOD2/CARD15 mutation), 29 with UC, and 10 with no inflammatory bowel disease (IBD). were tested for MAP by polymerase chain reaction (specific IS900 fragment) and blood culture. RESULTS:MAP DNA was detected in all original blood samples and 8-wk blood cultures (CD, UC and non-IBD). Positive MAP DNA status was confirmed by dot blot assays. All 69 cultures were negative by acid-fast Ziehl-Neelsen staining. Viable MAP, in spheroplast form, was isolated from the 18-mo blood cultures of all 30 CD patients, one UC patient, and none of the non-IBD controls. No association was found between positive MAP cultures and use of immunosuppressive drugs or CD-associated single nucleotide polymorphisms. CONCLUSION:MAP is widely present in our area and MAP DNA can be recovered from the blood of CD, UC and non-IBD patients. However, MAP spheroplasts were only found in CD patients.

SUBMITTER: Mendoza JL 

PROVIDER: S-EPMC2945487 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn's disease.

Mendoza Juan L JL   San-Pedro Amparo A   Culebras Esther E   Cíes Raquel R   Taxonera Carlos C   Lana Raquel R   Urcelay Elena E   de la Torre Fernando F   Picazo Juan J JJ   Díaz-Rubio Manuel M  

World journal of gastroenterology 20100901 36


<h4>Aim</h4>To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis (MAP) in patients with inflammatory bowel disease [Crohn's disease (CD) and ulcerative colitis (UC)].<h4>Methods</h4>Thirty patients with CD (15 with at least one NOD2/CARD15 mutation), 29 with UC, and 10 with no inflammatory bowel disease (IBD). were tested for MAP by polymerase chain reaction (specific IS900 fragment) and blood culture.<h4>Results</h4>MAP DNA was detected in all original blood s  ...[more]

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