Detection of microRNAs in dried serum blots.
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ABSTRACT:
SUBMITTER: Patnaik SK
PROVIDER: S-EPMC2947447 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Publications
Detection of microRNAs in dried serum blots.
Patnaik Santosh Kumar SK Mallick Reema R Yendamuri Sai S
Analytical biochemistry 20100807 1
We observed the preservation of microRNAs in unrefrigerated dried serum blots. Preservation was not adversely affected by drying or storing at 37, 45, or 60°C instead of room temperature, but it was harmed when blots were dried incompletely before storage. Preservation of microRNAs in serum was not diminished if, instead of being kept frozen at -80°C, it was stored as dried blots at room temperature for 5 months or at 37°C for 4 weeks. Thus, dried blots can be a convenient and safer way to save, ...[more]
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