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Long range dynamic effects of point-mutations trap a response regulator in an active conformation.

ABSTRACT: When a point-mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point-mutations, distant from an essential highly dynamic kinase recognition loop in the response regulator Spo0F, lock this loop in an active conformation. This 'conformational trapping' results in functionally hyperactive Spo0F. Consequently, point-mutations are seen to affect functionally critical motions both close to and far from the mutational site.

SUBMITTER: Bobay BG

PROVIDER: S-EPMC2949504 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Long range dynamic effects of point-mutations trap a response regulator in an active conformation.

Bobay Benjamin G BG Thompson Richele J RJ Hoch James A JA Cavanagh John J

FEBS letters 20100907 19

When a point-mutation in a protein elicits a functional change, it is most common to assign this change to local structural perturbations. Here we show that point-mutations, distant from an essential highly dynamic kinase recognition loop in the response regulator Spo0F, lock this loop in an active conformation. This 'conformational trapping' results in functionally hyperactive Spo0F. Consequently, point-mutations are seen to affect functionally critical motions both close to and far from the mu ...[more]

PMID: 20828564

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