Hemozoin-free Plasmodium falciparum mitochondria for physiological and drug susceptibility studies.
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ABSTRACT:
SUBMITTER: Mather MW
PROVIDER: S-EPMC2950219 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
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Hemozoin-free Plasmodium falciparum mitochondria for physiological and drug susceptibility studies.
Mather Michael W MW Morrisey Joanne M JM Vaidya Akhil B AB
Molecular and biochemical parasitology 20100730 2
Isolation of mitochondria of high purity and with intact enzymatic activities from malaria parasites has proven to be a major obstacle in characterizing the parasite mitochondrial physiology. We describe here an improved procedure for the isolation of a mitochondrially enriched preparation from the trophozoite stage of erythrocytic Plasmodium falciparum, combining disruption by N(2) cavitation and differential centrifugation with magnetic removal of hemozoin-associated material. These mitochondr ...[more]
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